Fig. 4

Multi-parametric dependence of PrLD-less TDP-43 self-assembly monitored by turbidimetry. A–D Fixed parameters were 5 μM PrLD-less TDP-43, pH 5.5, 1 mM TCEP, 25 °C. Final NaCl concentrations were 0 mM (A), 50 mM (B), 150 mM (C) and 300 mM (D), each containing 0% (w/v) PEG 8000 (black), 2.5% (w/v) PEG 8000 (red), 5% (w/v) PEG 8000 (blue) or 8% (w/v) PEG 8000 (pink), as indicated in each graph. The native protein was in 20 mM Tris–HCl, pH 8.0 (green). E–F Fixed parameters were 5 μM PrLD-less TDP-43, 5% (w/v) PEG 8000, 1 mM TCEP, 25 °C, at a final pH of 4.0 (E) or 7.0 (F). In all panels, final NaCl concentrations were 0 mM (solid line) or 150 mM (dash-dotted line), as indicated in each graph. The number of replicates was 3 (n = 3). Error bars: SEM. The dashed line indicates the threshold of turbidimetry above which the signal is significantly high to indicate visible phase separation