Fig. 3
From: Biophysical characterization of the phase separation of TDP-43 devoid of the C-terminal domain
Spectroscopic characterization of PrLD-less TDP-43 self-assembly in 0 mM NaCl. A Time course of PrLD-less TDP-43 self-assembly monitored with turbidimetry under native (red) and self-assembly promoting (black) conditions. The number of replicates was 3 (n = 3). Error bars: SEM. The dashed line indicates the threshold of turbidimetry above which the signal is significantly high to indicate visible phase separation. B Far-UV CD spectra of PrLD-less TDP-43 during self-assembly at the indicated time points. The spectrum of native PrLD-less TDP-43 is also shown. C Intrinsic Trp fluorescence spectra of PrLD-less TDP-43 during self-assembly at the indicated time points. The spectrum of native PrLD-less TDP-43 is also shown