Fig. 5

The influence of P2X7 receptor regulation on PI3K-Akt-GSMP3β and osteoclast function. A CCK-8 assay measured the absorbance of cells in various groups at 0, 12, 24, 36, and 48 h; B representative images of TRAP staining results; C spectrophotometer measured the absorbance of TRAP-stained cells at a wavelength of 405 nm; D resorption pit assay evaluated the differentiation and absorption ability of precursor cells in each group; E bar graph presenting the percentage of resorption pit area for each group; F western blot analysis determined the expression levels and grayscale values of MMP-9, CK, and NFATc1 in cells from each group; G bar graph illustrating the results of F-actin ring staining and the percentage of positive area. Bar = 50 μm. Compared with the Sh-P2X7 + DMSO group, **P < 0.01, ***P < 0.001; compared with the OE-P2X7 + DMSO group, ^##P < 0.01, ^###P < 0.001