Fig. 4

Regulatory role of P2X7 on the PI3K-Akt-GSK3β pathway. A Western blot analysis and grayscale value statistics of key factors in the PI3K-Akt-GSK3β signaling pathway phosphorylation levels in BMM cells transfected with P2X7 siRNA or overexpressed P2X7 lentivirus; B immunofluorescence staining and statistics of positive area percentage of key factors in the PI3K-Akt-GSK3β signaling pathway in different cell groups; C western blot analysis and grayscale value statistics of key factors in the PI3K-Akt-GSK3β signaling pathway phosphorylation levels in BMM cells transfected with P2X7 siRNA or overexpressed P2X7 lentivirus, and treated with activators or inhibitors of the PI3K-AKT-GSK3β signaling pathway; D immunofluorescence staining and statistics of positive area percentage of key factors in the PI3K-Akt-GSK3β signaling pathway in different cell groups. Bar = 50 μm. For (A, B), **P < 0.01, ***P < 0.001 compared with the Sh-NC group; ##P < 0.01, ###P < 0.001 compared with the OE-NC group. For (C, D), **P < 0.01, ***P < 0.001 compared with Sh-P2X7 + DMSO group; ^##P < 0.01, ^###P < 0.001 compared with OE-P2X7 + DMSO group