Fig. 3

Impact of P2X7 on osteoclast differentiation and resorption. A Western Blot analysis of P2X7 protein expression levels and grayscale value statistics in different cell groups; B RT-qPCR analysis of P2X7 mRNA expression levels and statistics in different cell groups; C CCK-8 assay to measure cell proliferation at 0, 12, 24, 36, and 48 h in different groups; D TRAP staining of cells in different groups; E statistics of TRAP activity; F measurement of resorption pits in different cell groups; G statistics of the percentage of area covered by resorption pits in different cell groups; H western blot analysis of protein expression levels and grayscale values of MMP-9, CK, and NFATc1 in different cell groups; I F-Actin ring staining results and statistics of immunofluorescence-positive cell counts. Bar = 50 μm. **P < 0.01, ***P < 0.001 compared with Sh-NC; ^##P < 0.01, ^###P < 0.001, compared with OE-NC. Cell experiments were repeated three times