Fig. 8

RUNX1, FUS and ELAVL1 can regulate the expression of circPTPN22. A and B ChIP-qPCR detection of GC cells ChIP-qPCR detection of PTPN22 promoter in GC cells may bind to the RUNX1 binding site, and IgG was used as a negative control. C Schematic diagram showing WT and Mut sequences of RUNX1 four putative binding sites on the PTPN22 promoter. D The combination of RUNX1 and PTPN22 was verified by dual luciferase reporter assay. E qRT-PCR assay to detect the expression of circPTPN22 and PTPN22 in GC cells after knockdown or overexpression of RUNX1. F Western blot detection of RUNX1 and PTPN22 protein expression in GC cells after RUNX1 knockdown. G Bioinformatic analysis of RNA-binding proteins associated with pre-circPTPN22. H RIP-qPCR detection of circPTPN22 expression in GC cells. I Expression of circPTPN22 and PTPN22 in GC cells after knockdown of FUS or ELAVL1. J and K After knocking down FUS or ELAVL1 in mRFP-GFP-LC3-labeled GC cells, the overexpression plasmid of circPTPN22 was added, and the autophagic flux was observed by confocal microscopy. Scale bar, 10 μM. L After knocking down FUS or ELAVL1, the overexpression plasmid of circPTPN22 was added, and the protein levels of E-cad, vimentin, P62/SQSTM1 and LC3 B in GC cells were detected by Western blot. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001