Fig. 3

circPTPN22 can directly bind miR-6788-5p. A and B Nuclear separation assay (A) and FISH assay (B) to detect the sublocalization of circPTPN22 in GC cells. Scale bar, 25 μM. C Bioinformatics detection of target miRNAs of circPTPN22. D qRT-PCR assay to detect the expression of miRNAs in GC cells after knockdown of circPTPN22. E and F Dual luciferase reporter gene assay to detect the binding of circPTPN22 and miR-6788-5p. G Anti-AGO2 RIP analysis of AGO2 protein expression detected by Western blotting. H qRT-PCR assay to detect the expression of circPTPN22 and miR-6788-5p. I RNA pulldown assay was performed in GC cells using biotin-labeled circPTPN22 probe, and then the enrichment of the indicated miRNAs was detected by qPCR analysis. J Correlation analysis of expression of circPTPN22 and miRNAs in 40 pairs of GC tissues. K Expression of miR-6788-5p in GC tissues. L Nuclear separation assay to detect the sublocalization of miR-6788-5p in GC cells. M FISH colocalization assay to detect the cellular sublocalization of circPTPN22 and miR-6788-5p. Scale bar, 25 μM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001