Fig. 1

circPTPN22 inhibits autophagy in GC cells. A Transmission electron microscope observation of the number of autophagosomes and autolysosomes in GC cells after knockdown and overexpression of circPTPN22. Scale bars are 5 μM and 2 μM, respectively. B Western blot assay to detect the protein levels of P62/SQSTM1 and LC3 B in GC cells after knockdown and overexpression of circPTPN22. C–F circPTPN22 was knocked down in mRFP-GFP-LC3-labeled MKN-1 and AGS cells, and circPTPN22 was overexpressed in MKN-45 and SGC-7901 cells. Confocal microscopy was used to observe the autophagic flux. C and E are representative confocal microscope images, and D and F are quantitative data. Scale bar, 10 μM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001