Fig. 2
α-KG alleviated oxidative stress and ferroptosis in H2O2-induced ATDC5 cells. A Assessing the changes in cell proliferation 24 h after α-KG treatment. B Measuring the effects of different α-KG concentrations on H2O2-induced ATDC5 cells. C, D Flow cytometry was used to assess the apoptosis rate in each group. E Fluorescence images of ROS in ATDC5 cells, where the green fluorescence intensity of DCFH-DA represents the ROS level. F ROS levels were analyzed using flow cytometry. G–I Levels of MDA, SOD, and GSH/GSSG in ATDC5 cells. J, K Alcian blue quantification and staining. L qRT-PCR analysis of ETV4, SLC7A11, and GPX4. M, N Western blot analysis and quantification of ETV4, SLC7A11, and GPX4. The statistical significance of the differences among groups was assessed using one-way ANOVA. Control vs H2O2, ###p < 0.001; H2O2 vs α-KG, *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3)