Fig. 3
ACE2 overexpression and interference affects the tight junction expression and mTOR pathway in IPEC-J2 cells. A Effect of ACE2 over-expression on ACE2, SLC6A19, AhR, 4EBP1, S6K1, ZO-1 and Occludin mRNA expression in IPEC-J2 cells. B Western blot results of ACE2, B0AT1, AhR, p-mTOR, ZO-1 and Occludin protein in IPEC-J2 cells over-expressing ACE2. C Trp contents of cell supernatant in control and overexpression groups. D Effect on the mRNA expression of ACE2, SLC6A19, AhR, 4EBP1, S6K1, ZO-1 and Occludin in IPEC-J2 cells expressing a control siRNA (NC) or ACE2 siRNA. E Western blot results of ACE2, B0AT1, AhR, p-mTOR, ZO-1 and Occludin protein of IPEC-J2 cells in NC or ACE2 siRNA group. F Trp contents of the supernatant in NC or ACE2 siRNA group. Data are shown as the mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001and ****P < 0.0001