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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway

Fig. 2

Trp repairs LPS-induced intestinal tight junction damage via ACE2/B0AT1 in IPEC-J2 cells. Effect of LPS (0–20 μg/mL) treatment on cell morphology (A) and number (B) of IPEC-J2 cells after 6 h (n = 6). Effect of LPS stimulation for 6 h on the morphology (C) and number (D) of IPEC-J2 cells after Trp pretreatment for 48 h (n = 6). E–K Effect of Trp on the mRNA expression of ACE2, SLC6A19, AhR, 4EBP1, S6K1, ZO-1and Occludin in IPEC-J2 cells under LPS stimulation (n = 3). L Western blotting results of ACE2, B0AT1, AhR, p-mTOR, ZO-1and Occludin upon treatment with Trp in LPS-induced IPEC-J2 cells (n = 3). M IPEC-J2 cells were treated with Trp and LPS for 24 h, and then the ZO-1, Occludin, mTOR and p-mTOR (green) were detected by immunofluorescence staining. Nuclei were counterstained with DAPI (blue). Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001. Scale bar shows 100 μm

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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