Fig. 1
ACE2/B0AT1 mediates Trp transport in IPEC-J2 cells. Effect of Trp (0–1.6 mM) treatments on cell morphology (A) and number (B) of IPEC-J2 cells after 48 h (n = 6). C Cell viability was measured using the CCK8 assay. All CCK8 values were normalized to the control group concentrations of Trp at 48 h. Cell supernatant (D) and intracellular (E) Trp content was measured by HPLC (n = 3). F, G Effect on the mRNA expression of ACE2 and SLC6A19 in IPEC-J2 cells treated for 48 h with Trp (0–0.4 mM), as determined by RT-qPCR (n = 3). H Western blotting analysis of ACE2 and B0AT1 protein upon Trp (0–0.4 mM) treatment for 48 h (n = 3). Data are expressed as the mean ± SEM. Scale bar shows 100 μm