Fig. 6

ERRα agonist alters mitochondrial functions in Aβ1-42 stimulated SH-SY5Y cells. a The structural formula of 1-[4-(3-tert-Butyl-4-hydroxyphenox) phenyl] ethan-1-one (ERRα agonist). b Cell viability of SH-SY5Y cells treated with ERRα agonist at indicated concentrations was determined by CCK8 assay. c Representative immunoblotting images of ERRα protein expression after agonist treatment in SH-SY5Y cells. d Representative fluorescence images of JC-1. JC-1 aggregates (red) and monomers (green) distributions after loading with JC-1 (1 μg/ml). Scale bars, 100 μm. e Ratios of the fluorescence intensities of JC-1 labelling. JC-1 aggregates and JC-1 monomers were measured by average cell fluorescence intensity by fluorescence microscopy. f Representative fluorescence images of end-point MMP in SH-SY5Y cells. DAPI (blue) and MitoTracker (red). Scale bars, 100 μm. g, h Quantification of MMP using MitoTracker-Red fluorescent probes. The MMP was measured by average cell fluorescence intensity by fluorescence microscopy (g) and fluorescence light intensity tested by fluoresce microplate reader (h). i Representative fluorescence images of mitochondrial ROS levels in SH-SY5Y cells. DAPI (blue) and MitoSox (red). Scale bars, 100 μm. j, k Quantification of mitochondrial ROS levels using MitoSox-Red fluorescent probes. The ROS level was measured by average cell fluorescence intensity by fluorescence and fluorescence light intensity tested by fluoresce microplate reader. e, g, j Data were presented as the mean ± S.E. The experiment had three independent biological replicates (Kruskal–Wallis test); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. b, h, k Data were presented as the mean ± S.E. The experiment had three independent biological replicates (One-way ANOVA); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001