Fig. 5
From: SGLT2 inhibitor promotes mitochondrial dysfunction and ER-phagy in colorectal cancer cells
iSGLT2 promotes autophagic flux and ER stress in CRC. Representative fluorescent images and cytofluorimetric detection of (A, B) lysosomes, (C,D) autophagy and (E) immunoblotting analysis with cropped blots of LC3B II/I ratio in HCT 116 cell lines. Representative fluorescent images and cytofluorimetric detection of (F, G) ER stress and immunoblotting analysis with cropped blots of (H) PERK, (I) CHOP and (J) ATF6 protein levels in HCT 116 cells treated with 50 µM iSGLT2 for 72 h (iSGLT2). Control cells (Ctr) were maintained in complete culture medium with the corresponding volume of HBSS-10 mM Hepes. Scale bars = 100 µm. M = molecular weight markers, lane 1 = Ctr, lane 2 = iSGLT2. Western blotting results are expressed as arbitrary units (AU). *p < 0.05 vs. Ctr; †p < 0.01 vs. Ctr; ‡p < 0.001 vs. Ctr, by unpaired Student’s t-test