Fig. 2
From: SGLT2 inhibitor promotes mitochondrial dysfunction and ER-phagy in colorectal cancer cells
iSGLT2 affects glucose metabolism in CRC. Evaluation of (A) glucose, (B) lactate, (C) ATP and (D) NAD+/NADH levels and immunoblotting analysis with cropped blots of (E) AMPK, (F) p-AMPK and (G) p-AMPK/AMPK ratio in HCT 116 cells treated with 50 µM iSGLT2 for 72 h (iSGLT2). Control cells (Ctr) were maintained in complete culture medium with the corresponding volume of HBSS-10 mM Hepes. M = molecular weight markers, lane 1 = Ctr, lane 2 = iSGLT2. Western blotting results are expressed as arbitrary units (AU). *p < 0.05 vs. Ctr; †p < 0.01 vs. Ctr; ‡p < 0.001 vs. Ctr, by unpaired Student’s t-test