Fig. 7
LIMA1 regulates mitochondrial autophagy and reduces its stability by interacting with PINK1. A Graphical representation of three-dimensional structures of the interaction model of LIMA1 with PINK1. B Co-IP analysis of the endogenous interaction of LIMA1 and PINK1 in LX2. C The co-localization between LIMA1 (green) with PINK1 (red) was analyzed by confocal microscopy in LX2. Scale bar = 10 μm. D qRT-PCR of PINK1 mRNA in LX2 with LIMA1 overexpression. Expression of PINK1 mRNA was quantified relative to β-actin. E qRT-PCR of PINK1 mRNA in LX2 with LIMA1 knockdown. F Western blot of PINK1 and Parkin in LIMA1-overexpressing plasmid-transfected LX2. G Western blot of PINK1 and Parkin in LIMA1-knockdown LX2. H LX2 overexpressing LIMA1 were treated with CHX to inhibit protein synthesis, and PINK1 protein turnover was analyzed over time. I Similarly, LX2 with knockdown of LIMA1 were also treated with CHX and to measure PINK1 protein levels. J Western blot of PINK1 and Parkin in LTH-sEVshCtr or LTH-sEVshLIMA1 treated LX2. All data were expressed as the means ± SD of at least 3 independent experiments, ns: no significance; *P < 0.05; **P < 0.01; ***P < 0.001