Fig. 2

LTH-sEV induce LX2 activation and LIMA1 expression. A Schematic diagram of the sEV stimulation experiment in vitro. B Immunofluorescence staining of α-SMA (green) and LIMA1 (red) in LX2 after accepting LTH-sEV. Scale bar = 20 μm. C-F qRT-PCR of LIMA1, COL1A1, COL3A1 and α-SMA mRNA in LX2 treated with LTH-sEV. G Western blot of LIMA1, COL1A1, COL3A1 and α-SMA in LX2 treated with LTH-sEV. H LX2 treated with LTH-sEV was detected by CCK-8 assay as indicated. I-L qRT-PCR of LIMA1, COL1A1, COL3A1 and α-SMA mRNA expression in LX2 treated with L02-sEV. M Western blot of LIMA1, COL1A1, COL3A1 and α-SMA expression in LX2 treated with L02-sEV. N LX2 treated with L02-sEV was detected by CCK-8 assay as indicated. All data were expressed as the means ± SD of at least 3 independent experiments, ns: no significance; *P < 0.05; **P < 0.001