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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression

Fig. 6

LINC00330 can inhibit the expression of CCL2 and the activation of its downstream signaling pathway. A KEGG analysis of the physiological pathways associated with the main regulation of LINC00330. B, C The top 30 genes downregulated by LINC00330 were “pathway” enriched according to the Reactome database. D The subcellular localization of CCL2 and LINC00330 was determined by RT‒PCR after cytoplasmic nucleus isolation. E Silver staining analysis after the LINC00330 RNA pulldown experiment. F The interaction between CCL2 and LINC00330 was detected by WB after the RNA pulldown experiment. G The interaction between CCL2 and LINC00330 was detected by RT‒PCR after RIP. H Pearson correlation analysis was used to analyze the correlation between LINC00330 and CCL2 expression. I WB was used to detect the effect of LINC00330 overexpression or knockdown on CCL2 protein expression in EC109 cells. J, K The effect of LINC00330 overexpression or knockdown in ESCC cells on the CCL2/CCR2 axis and its downstream signaling pathway was detected via RT‒PCR. L, M The effect of LINC00330 overexpression or knockdown on the CCL2/CCR2 axis and its downstream signaling pathway in ESCC cells was detected via WB. The figure shows the mean ± SD of the statistical results from three independent experiments; * P < 0.05, ** P < 0.01, *** P < 0.001

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