Fig. 4

Nrf-2 is involved in FGFR2-dependent response to anti-ER drugs. A MCF7 and B T47D cells were cultured in 3D Matrigel® for 10–12 days and 14 days, respectively, with FGF7 (50 ng/ml), ± 4-OHT (1 μM), ± Fulv (100 nM), ± K67 (500 nM). Representative images were taken (scale bar 100 μm), colonies were measured and analysed with ImageJ software. Quantitative data are presented as a relative ratio to CTR/non‐treated wild‐type cells, mean ± SD (n = 3), *P < 0.05. Statistical comparisons were made using 2-tailed Student’s t-test. C T47D and T47D Nrf-2↑ cells were cultured in 3D Matrigel® for 14 days with FGF7 (50 ng/ml) ± 4-OHT (1 μM) ± Fulv (100 nM). Representative images were taken (scale bar 100 μm), colonies were measured and analysed with ImageJ software. All quantitative data are presented as a relative ratio to CTR/non‐treated wild‐type cells, mean ± SD (n = 3), *P < 0.05. Statistical comparisons were made using 2-tailed Student’s t-test