Fig. 2

FGF7-induced autophagy abrogates the effect of anti-ER drugs. A MCF7 and B T47D cells were cultured in 3D Matrigel® for 10–12 days and 14 days, respectively, with FGF7 (50 ng/ml), ± 4-OHT (1 μM), ± Fulv (100 nM), ± CQ (1 μM). C T47D cells were cultured in 3D Matrigel® for 14 days with FGF7 (50 ng/ml) ± 4-OHT (1 μM), ± Fulv (100 nM), ± SBI-0206965 (750 nM). Representative images were taken (scale bar 100 μm), colonies were measured and analysed with ImageJ software. All quantitative data are presented as a relative ratio to CTR/non‐treated wild‐type cells, mean ± SD (n = 3), *P < 0.05 and **P < 0.01. All statistical comparisons were made using 2-tailed Student’s t-test