Fig. 5

Palmitic acid fate in LSECs. A The relative levels of acylcarnitines in LSECs incubated for 2 h with BSA (3.3 µM) or PA-BSA (20/3.3 µM) in KHB supplemented with glucose (2.5 mM) and l-carnitine (50 µM) normalised to the controls (without BSA and PA-BSA). Data are shown as means ± SDs from four independent experiments; *,#p < 0.05. B The OCR measured in LSECs in KHB supplemented with glucose (2.5 mM), l-carnitine (50 µM) and PA-BSA (20 µM/3.3 µM), untreated or treated with ETO (4 µM) and followed by DMSO or UK-5099 (10 µM) and CB-839 (5 µM), then FCCP (5 µM) and R/A (1/1 µM). Changes in basal respiration (90 min after ETO addition) and the maximal OCR were calculated from the kinetic data. Data are shown as means ± SEMs from three independent experiments (with three to four replicates per experiment). C The OCR measured in LSECs in KHB supplemented with glucose (2.5 mM), l-carnitine (50 µM) and BSA (3.3 µM), untreated or treated with ETO (4 µM) and followed by DMSO or UK-5099 (10 µM) and CB-839 (5 µM), then FCCP (5 µM) and R/A (1/1 µM). Changes in basal respiration (90 min after ETO addition) and the maximal OCR were calculated from the kinetic data. Data are shown as means ± SEMs from three independent experiments (with three to four replicates per experiment); #p < 0.05. D Relative levels of acylcarnitines in LSECs pre-incubated for 15 min with ETO (4 µM) and then incubated for 2 h with BSA (3.3 µM) or PA-BSA (20/3.3 µM) in KHB supplemented with glucose (2.5 mM) and l-carnitine (50 µM) normalised to the controls without ETO. Data are shown as means ± SDs from four independent experiments; *p < 0.05 compared with the control; #p < 0.05. E The CAT activity of LSECs, HAECs, MDA-MB-231 cells and hLMVECs. Data are shown as means ± SDs from three independent experiments. F Free FA concentration in FAO buffer (KHB supplemented with glucose [2.5 mM] and l-carnitine [50 µM]) with or without BSA (3.3 µM) after 2 h of incubation with or without LSECs. Data are shown as means ± SDs from five independent experiments; #p < 0.05