Fig. 1

Assessment of oxidative phosphorylation and glycolysis in LSECs. A A heat map of the relative abundance (emPAI%) of selected proteins identified in LSECs related to the TCA cycle, ETC, FAO in mitochondria, MAS, glutamine metabolism (G), peroxisomes and FAO in peroxisomes (PX) and glycolysis. The data are derived from eight replicates. B A representative image of LSEC mitochondria. Mitochondria (green) were labelled with MitoTracker Green FM, and nuclei (blue) were labelled with Hoechst 33342. C Changes in ATP concentration in LSECs treated for 1 h with DMSO (as a vehicle), oligomycin (O; 1 µg/mL) or IAA (20 µM). Data are shown as means ± SEMs from four independent experiments (with three replicates per experiment). Significance was tested using one-way ANOVA (A, B); *p < 0.05. D The OCR (grey) and ECAR (blue) in LSECs measured using the MST; the mitochondrial function parameters were calculated from the kinetic data. Data are shown as means ± SEMs from three independent experiments (with 5–6 replicates per experiment). E The ECAR (blue) and OCR (grey) in LSECs measured using the GST; the glycolytic function parameters were calculated from the kinetic data. Data are shown as means ± SEMs from three independent experiments (with 5–6 replicates per experiment). F Schematic presentation of glycolysis and oxidative phosphorylation with molecular targets for applied inhibitors (HK, hexokinase). The image was created using BioRender.com