Fig. 6
Application of the single-vector dCasRx translation enhancement tool in a mouse kidney stone model. A To package into adenoassociated viral vectors, the dCasRx-eIF4GI fusion protein and sgRNA complementary to DNA sequence of related genes was inserted into the consent skeleton containing the CMV and U6 promoters to form the plasmid dCasRx-eIF4GI-sgRNA. B Flow chart of animal model construction. C The mRNA expression level of FTH1 were detected using qRT–PCR analysis between Neg. stone model and SV-FTH1 stone model. D, E The protein expression of FTH1, ASCL4, and GPX4 were verified using western blot and the bar graph shows the relative expression levels. F Two indicators of kidney injury, including BUN content and serum CRE content, were measured. G Two indicators of ferroptosis, including Fe2+ content and MDA content, were measured. H The images show the degree of renal expression for GPX4 and ASCL4 in two groups (magnification ×400) and bar graph shows relative protein levels. I The mitochondrial morphology alterations associated with ferroptosis were observed using transmission electron microscopy (scale bar,  μm or 1 μm). J Crystal deposition was observed. Data are presented as the means ± SEM from six independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, no significant difference, represents P > 0.05