Fig. 7
From: NLRP4E regulates actin cap formation through SRC and CDC42 during oocyte meiosis

NLRP4E directly binds and phosphorylates SRC. A, B Immunofluorescence analysis showing that NLRP4E knockdown (KD) significantly reduced the intensity of actin filaments at the cap region; injected SRC-WT-EGFP mRNA significantly elevated the intensity. DNA is shown in blue, SRC-WT-EGFP is in green, and actin filaments (phalloidin) are in red. C–E In vitro phosphorylation assay and western blot showing that purified NLRP4E-flag could dose-dependently phosphorylate SRC. Scale bar, 20 μm. Different lower-case letters indicate significant differences between the two groups