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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Methylated lncRNAs suppress apoptosis of gastric cancer stem cells via the lncRNA–miRNA/protein axis

Fig. 3Fig. 3Fig. 3

Roles of methylated lncRNAs in GCSCs. A Expression profiles of six lncRNAs (PAPPA-AS1, PSMA3-AS1, MIR22HG, LINC00342, LINC01410, and LINC00680) in GCSCs. B Validation of silencing of lncRNAs in GCSCs. C Detection of the expressions of stemness genes in GCSCs with lncRNAs-siRNA. D Cell viability for PSMA3-AS1 or MIR22HG silencing GCSCs. E Cell cycle analysis of PSMA3-AS1 or MIR22HG-silenced GCSCs. F Caspase-3/7 assay for PSMA3-AS1 or MIR22HG silencing GCSCs. G Prediction of the methylation sites of lncRNAs. H The m6A levels of lncRNAs between GCSCs and GCNSCs. I The m6A levels of METTL3-silenced GCSCs with site-specific methylation of lncRNAs. J Cell viability for METTL3-silenced GCSCs with site-specific methylation of lncRNAs. K Cell cycle analysis of METTL3-silenced GCSCs with site-specific methylation of lncRNAs. L Caspase-3/7 assay for METTL3-silenced GCSCs with site-specific methylation of lncRNAs. M Western blot for stemness genes of METTL3-silenced GCSCs with site-specific methylation of lncRNAs. N The percentage of tumorsphere formation of METTL3-silenced GCSCs with site-specific methylation of lncRNAs. O Tumorsphere formation assay of a single cell from METTL3-silenced GCSCs with methylated lncRNAs. **, p < 0.01

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