Fig. 4

Western blot and relative densitometries of FASN (a, b), ACSS1 (a, c), and precursor and cleaved SREBP1 (a, d, e) expression in AGC1^+/− and AGC1^+/+ neurospheres; GAPDH was used for endogenous normalization. Confocal microscopy images (f) in neurospheres; nuclei were labeled with DAPI. Scale bar 50 µm; 60 × objective. Reverse transcription real-time PCR analysis (g). GAPDH were used as endogenous controls. Values are mean ± standard deviation (SD) of at least three independent experiments; ***p < 0.001, **p < 0.01, *p < 0.05, compared with AGC1^+/+ control neurospheres Student’s t-test