Fig. 6

Cross-validation results for the RNA specificity in the crRNA pool of the constructed CRISPR system. a–f Specificity cross-validation results for CRH1 to CRH6, respectively, against various HPV types. DNA templates for HPV 16–45 were added at concentrations of 10⁴ copies/μL each for the cross-validation. N negative control. The experimental results demonstrate that each crRNA shows no cross-reaction with HPV types other than its corresponding type