Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content
Fig. 10 | Cellular & Molecular Biology Letters

Fig. 10

From: A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay

Fig. 10

Comparison of experimental results between the H-MRC12a combined detection system and a commercially available QPCR genotyping kit for detecting 8 clinical HPV 18-positive samples. Clinical samples were heat-inactivated and subjected to nucleic acid extraction using the nanomagnetic bead method. The extracted nucleic acids were then incubated in the H-MRC12a combined detection system for 40 min (Multiple RPA incubation for 20 min, followed by CRISPR incubation for an additional 20 min) and immediately observed under 300 nm UV light. a Fluorescence curves and CT values obtained from the commercially available QPCR genotyping kit for the 8 clinical samples. b Real-time fluorescence signals (recorded using the Q160 LongGene portable QPCR instrument) of the H-MRC12a dual detection system for the clinical samples and negative control after 20 min of incubation in the CRISPR step. c Photographs taken under UV light of the 8 clinical samples incubated for 40 min in the H-MRC12a detection system. S18-1~S18-8: Identification numbers of the 8 HPV 18-positive clinical samples; CRH1~6: Tubes containing crRNA specific for HPV 16, 18, 31, 33, 35, and 45, respectively, for genotyping detection; NC: Negative control

Back to article page