Fig. 10

Confirmation of hub MitoDEG expression and the key role of mitochondrial dysfunction in the pathogenesis of SCM. A mRNA expression of the hub MitoDEGs between the control and SCM groups. B Analysis of HL-1 mitochondrial metabolism with Seahorse XFe96 Analyzer. OCR was monitored continuously at baseline and after the addition of oligomycin (2 mM), FCCP (1 mM), and R/A (0.5 mM). C–H Basal respiration, maximal respiration, nonmitochondrial oxygen consumption, spare respiratory capacity, proton leak, and ATP production levels. Mitochondrial metabolism is impaired in the SCM group. OCR, oxygen consumption rate; FCCP, carbonyl cyanide-4-phenylhydrazone; R/A, rotenone and antimycin A (N = 8 independent cell samples per group). *p < 0.05, **p < 0.01, ***p < 0.001