Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content
Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Increased serum extrachromosomal circular DNA SORBS1circle level is associated with insulin resistance in patients with newly diagnosed type 2 diabetes mellitus

Fig. 4

Expression of eccDNA SORBS1circle in HG/PA-treated HepG2 cells. A, B The protein levels of P-Akt S473 and T-AKT in HepG2 cells treated with 25.0 mM glucose (HG) and 0.5 mM palmitate (PA) were evaluated by western blotting. These HepG2 cells were treated with 100 nM insulin for 20 min before being harvested. C qPCR was performed for the determination of intracellular eccDNA SORBS1circle level in HG/PA-treated HepG2 cells. D Visualization of eccDNA SORBS1circle expression in control and HG/PA-treated HepG2 cells using FISH assay. The complete image of FISH assay was shown in Additional file 1: Fig. S1. Bar = 10 μm. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01

Back to article page