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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: IP3R1-mediated MAMs formation contributes to mechanical trauma-induced hepatic injury and the protective effect of melatonin

Fig. 5

Melatonin suppressed mitochondrial calcium overload, increased mitochondrial membrane potential and improved mitochondrial function in TS-induced hepatocytes. A, B Representative confocal images of mitochondrial Ca2+ concentration in primary hepatocytes stained by Rhod-2 dye at × 600 magnification and quantification of fluorescence intensity normalized by SS. C Representative traces of ionomycin-induced changes of mitochondrial Ca2+ concentration (n = 3). D Quantification of the relative increment of fluorescence intensity in mitochondrial Ca2+ concentration after ionomycin stimulation normalized by SS. E Quantification of fluorescence intensity in cytosolic Ca2+ concentration stained by Fura-2 AM dye normalized by SS. F Representative traces of ionomycin-induced changes of cytosolic Ca2+ concentration (n = 3). G Quantification of the relative increment of fluorescence intensity in cytosolic Ca2+ concentration after ionomycin stimulation normalized by SS. H, I Representative flow cytometry results and statistical analysis of mitochondrial membrane potential by JC-1 stanning in primary hepatocytes. J, K Oxygen consumption rate (OCR) measured by Seahorse and quantitative statistical analysis of OCR (n = 3). All of the values are shown as the means ± SEM. n = 6 in each group. **P < 0.01 vs SS; ##P < 0.01 vs TS

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