Fig. 5
From: Cadmium promotes nonalcoholic fatty liver disease by inhibiting intercellular mitochondrial transfer
The GSK-3β-Tau pathway mediates cadmium-induced disruption of microtubules. AML12 cells were treated with 2.5, 5, and 10 μM Cd for 6 h. Levels of GSK-3β and Tau were determined using western blotting (A). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Based on treatment with GSK3B siRNA or NC siRNA for 24 h, AML12 cells were treated with or without Cd for 6 h. TOMM20 and Tubulin-Tracker-Red co-staining to detect mitochondrial transfer (B). Scale bar = 25 μm. Oil red O (C) and BODIPY (D) were used to observe intracellular lipid droplets. Scale bar = 25 μm. TG and TC contents were detected (E, F). Levels of CPT1and ACSL1 were determined using western blotting (G). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, #P < 0.05, ##P < 0.01