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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice

Fig. 5

Transcriptional activation of BZW1 by CEBPB promotes metabolic reprogramming of macrophages. BMDM were induced with Ova-IC and further treated with sh-NC or sh-CEBPB. A ECAR and OCR traces for BMDM. B The ECAR/OCR ratio in BMDM. C The glucose uptake capacity of BMDM was measured using a glucose uptake assay. D Glycolysis-related protein HK2 and LDHA expression in BMDM were measured using western blot assays. BMDM induced with Ova-IC were further treated with sh-CEBPB + oe-NC or sh-CEBPB + oe-BZW1. E BZW1 expression in BMDM after co-transfection. F ECAR and OCR traces for BMDM. G The ECAR/OCR ratio in BMDM. H The glucose uptake capacity of BMDM was measured using a glucose uptake assay. I Glycolysis-related protein HK2 and LDHA expression in BMDM were measured using western blot. J Expression of IL-1β, IL-6, and TNF-α in BMDM by RT–qPCR. K Expression of IL-1β, IL-6, and TNF-α in BMDM in response to 2DG by RT–qPCR. Data are presented as means ± SD and representative of three independent experiments (*p < 0.05). Data were statistically analyzed using an unpaired t-test (E, G, H), one-way ANOVA (B, C), or two-way ANOVA (A, D, F, I–K)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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