Fig. 3

Effects of the CRISPR–dCas9–KRAB system on cell viability after 24, 48, and 72 h compared with the sgRNA control group. CCK-8 was used to detect cell proliferation viability. There were statistical differences between the two groups in A–C, and the P-value was less than 0.01. There was no statistical difference between the two groups in D. ANOVA was used for statistics. Flow cytometry was used to detect cell apoptosis rate (%). There were statistical differences between the two groups in MG-63, Saos-2, U-2 OS, and hFOB 1.19. The P-value was less than 0.01. ANOVA was used for statistics. E Representative pictures of apoptosis. F Statistical histograms