Table 1 The primers and antibodies used in this study
From: Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells
Primers | |
LDHA | Forward: TTGTTGGGGTTGGTGCTGTTG |
Reverse: AAGAGCAAGTTCATCTGCCAAG | |
COX4 | Forward: CGATCACCTTGACGGACGAG |
Reverse: TCTTCTCATAGTCCCAGCGC | |
Antibodies for western blot | |
LDH | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
COX4 | CST, COX IV (3E11) rabbit mAb, #4850 |
DRP1 | Proteintech, DRP1 polyclonal antibody, 12957-1-AP |
MFN2 | Proteintech, MFN2 polyclonal antibody, 12186-1-AP |
MFF | Abcam, anti-MFF antibody, ab129075 |
PINK1 | Proteintech, PINK1 polyclonal antibody, 23274-1-AP |
PARKIN | Proteintech, PARK2/Parkin monoclonal antibody, 66674-1-Ig |
GAPDH | Proteintech, GAPDH polyclonal antibody, 10494-1-AP |
β-actin | Proteintech, beta actin polyclonal antibody, 20536-1-AP |
Antibodies for immunofluorescence staining | |
LDH | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
COX4 | CST, COX IV (3E11) rabbit mAb, #4850 |
ldh | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
Cox4 | CST, COX IV (3E11) rabbit mAb, #4850 |
DRP1 | Proteintech, DRP1 (C-terminal) polyclonal antibody, 12957-1-AP |
VDAC1 | Proteintech, VDAC1/Porin monoclonal antibody, 66345-1-Ig |
Drp1 | Proteintech, DRP1 (C-terminal) polyclonal antibody, 12957-1-AP |
Vdac1 | Proteintech, VDAC1/Porin monoclonal antibody, 66345-1-Ig |
Antibodies for immunohistochemical staining | |
ldh | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
cox4 | CST, COX IV (3E11) rabbit mAb, #4850 |