Fig. 4

RPL21 upregulates LAMP3 expression by activating TFEB. A TFEB, p-TFEB (Ser211), RPL21, and LAMP3 protein expression in the indicated CRC cells was detected by western blotting. B The TFEB protein expression in cytosolic and nuclear of the indicated CRC cells was detected by western blotting. The quantification of cytosolic TFEB was normalized to GAPDH, the quantification of nuclear TFEB was normalized to H3. C The localization of TFEB (green) in the indicated CRC cells was observed by IF staining (scale, 20 μm). D The transcription of LAMP3 by TFEB was verified by dual-luciferase reporter gene assay in HEK293T cells. Mean ± SD, n = 3, ***p < 0.001, Student’s t-test. E The transcription of LAMP3 in the indicated CRC cells was verified by dual-luciferase reporter gene assay. Mean ± SD, n = 3, ***p < 0.001, **p < 0.01, *p < 0.05, Student’s t-test