Table 1 Primer sequences used in the study
Gene | Forward/reverse | Sequence (5′–3′) |
|---|---|---|
DGKK | Forward | AAGAAACAGTCAGGGTCAAC |
Reverse | AGGATGGAATGGTGCTAATG | |
NOX1 | Forward | ATAGCAGAAGCCGACAGG |
Reverse | CCACCAATGCCGTGAATC | |
NOX2 | Forward | TAAGATAGCGGTTGATGGG |
Reverse | CAGATTGGTGGCGTTATTG | |
NOX3 | Forward | TTGGCGTGTTCTTCTGTG |
Reverse | TCCTGGTGGAGTTCTTTG | |
NOX4 | Forward | GACTTGGCTTTGGATTTCTG |
Reverse | TCTGAGGGATGACTTATGAC | |
NOX5 | Forward | TGCACTGGGCAAGAATGAC |
Reverse | AGCAGCCACTTTCTGGAAC | |
DUOX1 | Forward | AAGTCTCGCCTTATGTTC |
Reverse | ATCTTCCCATGTCAGTTC | |
DUOX2 | Forward | GAACATCGCTGTGTATGAGTG |
Reverse | TTCTCCCGAATCCAGTAGTTG | |
IL6 | Forward | GCACCTCAGATTGTTGTTG |
Reverse | AGTGTCCTAACGCTCATAC | |
IL1B | Forward | ATCAGCCAGGACAGTCAG |
Reverse | GAAGCGGTTGCTCATCAG | |
TNF | Forward | GGTATGAGCCCATCTATCTG |
Reverse | AGGGCAATGATCCCAAAG | |
GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT |
Reverse | GGCTGTTGTCATACTTCTCATGG | |
Dgkk | Forward | GGAATTACTGCAACGCTCTTAC |
Forward | AACCAAAGATTGCCACAACC | |
Il6 | Forward | TGGAGCCCACCAAGAACGATAG |
Reverse | TGTCACCAGCATCAGTCCCAAG | |
Il1b | Forward | GCATCCAGCTTCAAATCTC |
Reverse | ACACCAGCAGGTTATCATC | |
Tnf | Forward | GTGCTCAGAGCTTTCAAC |
Reverse | ACTCTCCCTTTGCAGAAC | |
Gapdh | Forward | CTGCCCAGAACATCATCC |
Reverse | CTCAGATGCCTGCTTCAC |