Fig. 3

RAP2A was the target of miR-33a-5p. A Binding sequences of miR-33a-5p at the RAP2A 3′-UTR mRNA are displayed. B, C Relative luciferase activity was determined after co-transfection with either miR-33a-5p mimics or miR-NC and either WT RAP2A or MUT RAP2A in SW1990 and PANC-1 cells. D, E RT-qPCR and western blotting were performed to determine RAP2A expression level in SW1990 and PANC-1 cells following transfection with miR-33a-5p mimics or miR-NC. F, G RNA RIP experiments were performed in SW1990 and PANC-1 cells and the co-precipitated RNA was analyzed by RT-qPCR. **p < 0.01, ***p < 0.001 vs. miR-NC group