Fig. 5

TGF-β overexpression rescues the miR-296-5p-induced inhibition of tumor metastasis in NPC cells via EMT. a Transwell assays showed that exogenous TGF-β rescued the miR-296-5p-induced inhibition of metastasis in NPC cells. Scale bar 100 µm. b Immunofluorescence showed exogenous TGF-β rescued the miR-296-5p-induced transition to epithelial-like cells. Scale bar 100 µm. c Western blotting confirmed exogenous TGF-β rescued the expression of the EMT-related proteins in NPC cells. d The relative expression levels of the EMT-related marker gene proteins with gray-scale analysis. *p < 0.05)