Fig. 4

MiR296-5p suppresses TGF-β expression by directly targeting its 3ʹ-UTR. a Western blotting showed miR-296-5p expression inversely affected TGF-β expression in NPC cells. b Real-time PCR showed that miR-296-5p expression affected the downstream gene expression of TGF-β in NPC cells. c The binding sites of miR-296-5p with wild-type or mutant TGF-β in 3ʹ-UTR. d Luciferase assays in 293T cells treated with both wild-type or mutant TGF-β vectors along with an miR-296-5p mimic, inhibitor or relevant NC