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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: RETRACTED ARTICLE: The novel circular RNA circ-CAMK2A enhances lung adenocarcinoma metastasis by regulating the miR-615-5p/fibronectin 1 pathway

Fig. 3

Circ-CAMK2A directly binds to miR-615-5p in LUAD cell lines. a, b Subcellular localization of circ-CAMK2A was determined by qRT-PCR and FISH assays. Nucleus was stained with DAPI. c, d Schematic diagram of circ-CAMK2A with three miR-615-5p binding sites predicted by RegRNA 2.0 online tool. e Luciferase reporter assay of circ-CAMK2A luciferase vector with wild-type or mutated miR-615-5p binding sites in A549 and HCC827 cells after co-transfection with control and miR-615-5p mimics, respectively. (F) RNA pull-down assay using oligo or circ-CAMK2A probe in A549 and HCC827 cells, followed by qRT-PCR analysis for miR-615-5p expression. g, h qRT-PCR analysis for miR-615-5p expression in LUAD tissues and cell lines. i Transwell migration and invasion assays in circ-CAMK2A-overexpressing A549 and circ-CAMK2A-silencing HCC827 cells. j qRT-PCR analysis for circ-CAMK2A and miR-615-5p expression in circ-CAMK2A-overexpressing A549 and circ-CAMK2A-silencing HCC827 cells. k Correlation between circ-CAMK2A and miR-615-5p expression in 58 LUAD tissues, as determined by Pearson’s correlation coefficient. **p < 0.01

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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