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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: RETRACTED ARTICLE: MicroRNA-582–3p negatively regulates cell proliferation and cell cycle progression in acute myeloid leukemia by targeting cyclin B2

Fig. 4

Upregulation of miR-582–3p induced cell proliferation inhibition and G2/M cell cycle arrest by targeting CCNB2. THP-1 cells were transfected with siCCNB2, siNC, miR-582–3p mimics + pcDNA3.1 or miR-582–3p mimics + pcDNA3.1-CCNB2. a The protein level of cyclin B2 expression was detected using western blot. b CCK8 assay was performed to evaluate cell proliferation at the indicated times following transfection. c THP-1 cells were stained with PI and subjected to flow cytometry analysis, followed by quantification of cell cycle distribution. Data are presented as means ± SD from three independent experiments. **p < 0.01, ***p < 0.001 vs. siNC; ##p < 0.01, ###p < 0.001 vs. miR-582–3p mimics + pcDNA3.1

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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